Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14.

نویسندگان

  • Wei Wang
  • Feng-Qing Wang
  • Dong-Zhi Wei
چکیده

A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 75 12  شماره 

صفحات  -

تاریخ انتشار 2009